Identification of Lactobacillus spp. on Basis Morphological, Physiological, and Biochemical Characteristic from Jawa Super Chicken Excreta

The use of antibiotic growth promoter as a feed additive for animals in Indonesia has been prohibited and regulated law, starting from January 1, 2018. Alternative replacement was investigatedand introduced to farms such probiotic. commonly used probiotic the genus Lactobacillus, Bifidobacterium , Bacillus. aim this study identify Lactobacillus strains isolates Jawa Super chicken excreta. Identification based on morphological, physiological, biochemical characteristics. sample collected Kalasan, Sleman, Yogyakarta. Each diluted 0.9% sterile saline plated MRS agar containing bromocresol purple. All plates were incubated at 37°C under anaerobic condition. Isolates identified morphology, physiology, biochemistry fermentation carbohydrate by using Analytical Profile Index (API 50 CHL). This showed that belong 2 categories fermentation, facultatively heterofermentative obligately heterofermentative. Not all can grow 10°C, but survive low pH. Based analysis L. brevis pentosus . found